Reporter

Part:BBa_K1093031

Designed by: Jakub Piątkowski   Group: iGEM13_Warsaw   (2013-09-21)


HBB+CsfGFP m12 (+B0034)

Human hemoglobin beta chain fused at C-terminus with the C-terminal fragment of superfolder green fluorescent protein. m12 mutated version of the C-terminal sfGFP fragment offers larger specificity at the price of lowers sensitivity (as opposed to m6 vaersion) Along with BBa_K1093020 and BBa_K1093021 it is intended as a part of a reporter system for acrylamide detection. Formation of adducts on the N-terminal valine of both hemoglobin chains is known to inhibit proper assembly of hemoglobin tetramer. Part already contains a strong RBS (BBa_B0034). The coding sequence has been codon optimised for E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 315
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None